Dr. Sigrid Milles

Sigrid Milles head

Leibniz Forschungsinstitut für Molekulare Pharmakologie (FMP) Berlin, Germany

E-mail: milles@fmp-berlin.de

Phone: ‭+49 30 94793‬


Research Area

Integrated Structural Dynamics 


Position and Status

Junior Group Leader


Scholarships and awards

2019 Paoletti Prize 2019 awarded by the national institute for structural biology (INSB) of the French national research center (CNRS)

2018 Receipient of an ERC (European research council) starting grant

2017 National competition for CNRS researcher positions in France: classed 1st in section 16 (Chimie et vivant) and 20 (Biologie moléculaire et structurale, biochimie)

2015 – 2017 EMBO (European Molecular Biology Organisation) long-term fellow

2016 Recipient of the “Young researcher ptize” of the French Biophysical Society

2010 – 2012 PhD fellowship from the Boehringer Ingelheim Fonds



University training and degree

2006 – 2008, Studies of Biochemistry at the University Paris VII – Denis Diderot, France; Mentor: Prof. Gilbert Richarme

2004 – 2009, Studies of Biophysics at the Humboldt University of Berlin, Germany, Diploma, Prof. Dr. Thomas Günther-Pomorski

 

Advanced academic qualifications

2011 - PhD in Biophysics, European Molecular Biology Laboratory (EMBL) and Ruperto Carola University of Heidelberg, Germany, Dr. Edward A Lemke

 

Postgraduate professional career

2022 - present - Junior Group Leader ‘Integrated Structural Dynamics’ at Leibniz Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany

2009 - 2022 - Team leader at Institut de Biologie Structurale (IBS), Grenoble, France

2017 - 2022 -  Researcher of the French national research center (CNRS, CRCN) at IBS, Grenoble, France

2014 - 2017  Postdoc at the Institut de Biologie Structurale, Grenoble, France Protein dynamics and flexibility by NMR group (Dr. Martin Blackledge)

2013 - 2014  Postdoc at the European Molecular Biology Laboratory, Heidelberg, Germany

2022 – present  Teaching of biological NMR Spectroscopy at Freie Universität Berlin, Germany

Participation at the university master course ‘Biophysical methods’ at Freie Universität Berlin, Germany

Participation in the lecture series ‘Experimental Biophysics’ at Humboldt- University of Berlin

2021 – 2022 Head of research axis ‘assembly, dynamics et reactivity’ at the Institut de Biologie Structurale (IBS), France

2020 – 2022 Member of the redaction committee of the scientific IBS newsletter

2020 – present Mentor in the ‘LEAP – Leadership and Excellence for Aspiring Postdoc’ program for EMBL’s female postdocs

2016 – 2018  Participation in lecture series for PhD and Master students at University of Grenoble, Alpes

2010 Organizing committee member for the 12th international PhD Symposium, Heidelberg, Germany

 

 

 

 

 

  1. Naudi-Fabra, S., Tengo, M., Jensen, M. R., Blackledge, M. & Milles, S. Quantitative Description of Intrinsically Disordered Proteins Using Single-Molecule FRET, NMR, and SAXS. Journal of the American Chemical Society 143, 20109-20121 (2021). https://doi.org/10.1021/jacs.1c06264
  2. Guseva, S., Milles, S., Jensen, M. R., Salvi, N., Kleman, J.-P., Maurin, D., Ruigrok, R. W. H. & Blackledge, M. Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly. Science Advances 6, eaaz7095 (2020). DOI: 10.1126/sciadv.aaz7095
  3. Desfosses, A., Milles, S., Jensen, M. R., Guseva, S., Colletier, J.-P., Maurin, D., Schoehn, G., Gutsche, I., Ruigrok, R. W. H. & Blackledge, M. Assembly and cryo-EM structures of RNA- specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication. Proceedings of the National Academy of Sciences 116, 4256-4264 (2019). https://doi.org/10.1073/pnas.1816417116
  4. Milles, S., Jensen, M. R., Lazert, C., Guseva, S., Ivashchenko, S., Communie, G., Maurin, D., Gerlier, D., Ruigrok, R. W. H. & Blackledge, M. An ultraweak interaction in the intrinsically disordered replication machinery is essential for measles virus function. Science Advances 4, eaat7778 (2018). DOI: 10.1126/sciadv.aat7778
  5. Milles, S., Jensen, M. R., Communie, G., Maurin, D., Schoehn, G., Ruigrok, R. W. H. & Blackledge, M. Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence Dependence. Angewandte Chemie International Edition 55, 9356-9360 (2016). https://doi.org/10.1002/anie.201602619 74CRC/TRR 186 – Sigrid Milles
  6. Milles, S., Mercadante, D., Aramburu, Iker V., Jensen, Malene R., Banterle, N., Koehler, C., Tyagi, S., Clarke, J., Shammas, Sarah L., Blackledge, M., Gräter, F. & Lemke, Edward A. Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors. Cell 163, 734-745 (2015). DOI: 10.1016/j.cell.2015.09.047
  7. Milles, S. & Lemke, E. A. Mapping Multivalency and Differential Affinities within Large Intrinsically Disordered Protein Complexes with Segmental Motion Analysis. Angewandte Chemie International Edition 53, 7364-7367 (2014). https://doi.org/10.1002/anie.201403694
  8. Milles, S., Huy Bui, K., Koehler, C., Eltsov, M., Beck, M. & Lemke, E. A. Facilitated aggregation of FG nucleoporins under molecular crowding conditions. EMBO reports 14, 178- 183 (2013). https://doi.org/10.1038/embor.2012.204
  9. Milles, S., Tyagi, S., Banterle, N., Koehler, C., VanDelinder, V., Plass, T., Neal, A. P. & Lemke, E. A. Click Strategies for Single-Molecule Protein Fluorescence. Journal of the American Chemical Society 134, 5187-5195 (2012). https://doi.org/10.1021/ja210587q
  10. Milles, S. & Lemke, Edward A. Single Molecule Study of the Intrinsically Disordered FG- Repeat Nucleoporin 153. Biophysical Journal 101, 1710-1719 (2011). DOI: 10.1016/j.bpj.2011.08.025